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Department of Chemitry

International Journal

Cytokine
년도 2020
학술지명 Cytokine
논문명 An adiponectin receptor agonist antibody stimulates glucose uptake and fatty-acid oxidation by activating AMP-activated protein kinase
게재권/집 vol.126/ Article Number154863
수록페이지
저자명 Jung D, Bucher F, Ryu S, Jeong Jong Won, Lee Beom Yong, Jeong Youngtae, Kim Minseok S, Oh Yong-Seok , Baek M.-C, Yoon J.H, Ye K
Link 관련링크 https://www.sciencedirect.com/science/article/pii/S1043466619302923?vi… 11558회 연결
Abstract

Adiponectin (Ad) is a representative adipocytokine that regulates energy homeostasis including glucose transport and lipid oxidation through activation of AMP-activated protein kinase (AMPK) pathways. Plasma levels of Ad are reduced in obesity, which contributes to type 2 diabetes. Therefore, agents that activate the Ad signaling pathway could ameliorate metabolic diseases such as type 2 diabetes. Here, we report the identification of a high-affinitive agonist antibody against Ad receptors. The antibody was selected by using phage display of human combinatorial antibody libraries. The selected antibody induced phosphorylation of the acetyl-CoA carboxylase (ACC) and AMPK in skeletal muscle cells and stimulated glucose uptake and fatty-acid oxidation (FAO) in myotubes. In addition, the antibody significantly lowered blood glucose levels during a glucose challenge in normal mice as well as basal blood glucose levels in a type 2 diabetic mouse model. Taken together, these results suggest that the agonist antibody could be a promising therapeutic agent for the treatment of metabolic syndrome such as type 2 diabetes.